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Fig. 2. Expression of ß_H segment 33 induces apoptosis in imaginal tissues. (A) Wild-type wing. (B) Notches (arrows) resulting from the expression of ßH33 under the control of the vg-Gal4 driver along the wing margin. (C) ßH33-induced notching is rescued by co-expression of the baculovirus p35 caspase inhibitor from a UAS-p35 transgene. Curiously, vein L5 (arrow) is incomplete in such wings. (D) Scanning electron micrograph (SEM) of a wild-type eye. (E) SEM of an eye resulting from the expression of ßH33 under the control of the GMR-Gal4 driver in the developing eye disc at 20°C, which produces an intermediate phenotype. (F) The rough eye phenotype shown in E is rescued by co-expression of the baculovirus p35 caspase inhibitor from a GMR-p35 transgene. (G) Live wild-type 3rd instar wing disc stained with acridine orange to reveal cells undergoing apoptosis. (H) Live 3rd instar wing disc expressing ßH33 under the control of the vg-Gal4 driver stained with acridine orange. Numerous dying cells are detected in the region of greatest ßH33 expression along the wing margin (arrows). (I) SEM of a typical eye resulting from the expression of the ß-spectrin C-terminal derivative ßspPH under the control of the GMR-Gal4 driver at 25°C. Only a mild phenotype composed of a few mispositioned bristles is seen despite abundant protein expression (Fig. 1C).

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