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Fig. 3. ßH segment 33 exhibits autonomous membrane localization. Sagittal confocal sections of salivary glands are shown in A-I. A parasagittal section in the plane of the lumenal surface is shown in J-L. All embryos are between stages 13 and 16. (A) A wild-type salivary gland stained for {alpha}-spectrin. (B) A wild-type salivary gland stained for ß-spectrin. (C) A wild-type salivary gland stained for ßH-spectrin. Inset, parasagittal image at the lumenal surface. (D-L) Salivary glands expressing ßH33 under the control of the fkh-Gal4 driver. Glands are costained for ßH33 with mAb 9E10 and anti-ß-spectrin (D-F), anti-ßH (G-I) or anti-DMoesin (J-L) antibodies. In each case staining for ßH33 is shown on the left (red in the merged image) with the second protein on the right (green in the merged image). Bars, 20 µm.





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