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Fig. 5. Overexpression of ßH segment 33 generates a distinct membrane domain associated with dynamin. (A-R) Costaining for ßH derivative ßHPH+5-3 using mAb 9E10 (left panel, red in merged image) and various membrane markers (right panel, green in merged image). All embryos are at stage 15. (A-F) Costaining for the apical transmembrane protein Stranded at second (Sas). (A-C) A parasagittal section at the level of the lumenal surface. (D-F) Sagittal section of the same gland. (G-I) Costaining for the apical cortical protein DMoesin. (J-L) Costaining for the endocytic protein clathrin. Clathrin puncta (see arrowheads) are seen in arrangements that suggest some association with the membrane protrusions. (M-O) Costaining for the endocytic protein amphiphysin. (P-R) Costaining for the protein dynamin (Shibire). (C',F',I',L',O',R') Comparable stainings of wild-type glands for the same antigen as in C, F, I, L, O and R, respectively. Bars, 20 µm.





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