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Fig. 4. Immunofluorescence microscopy to identify occludin and ZO-1 in the seminiferous epithelium of rat testes at the BTB site during CdCl2-induced BTB damage. (A-O) Immunofluorescence micrographs of cross sections of normal rat testes (A-C) and rat testis at 7 hours (D-F), 12 hours (G-I) and 24 hours (J-O) after CdCl2 treatment. Occludin appears as red fluorescence (Cy-3, white arrowheads in A), ZO-1 as green fluorescence (FITC, arrowheads in B). (C,F,I,L,O) Merged images of the corresponding immunofluorescence micrographs for occludin and ZO-1, in which co-localization appears orange (arrowheads in C). White broken lines in M-O indicate the approximate location of the basement membrane of the seminiferous epithelium. Scale bar: 20 µm.





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