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Fig. 1. Substrate dependence of dextran endocytosis. Rabbit osteoclasts cultured overnight on glass (A-D) or dentine (E-H) were incubated for 5 minutes with either FITC-labelled dextran-3000 (FD3) (A,E) or FITC-labelled dextran-70,000 (FD70) (C,G). Cells plated on glass are non-resorbing and endocytose both markers to a similar extent (A,C), whereas resorbing osteoclast plated on dentine only endocytose low-molecular-weight dextran 3000 (E,G). F-actin was visualized with rhodamine-conjugated phalloidin (B,D,F,H). The typical ring-like actin structure of resorbing osteoclasts is only visible in cells plated on dentine (F,H, arrow). The outline of the cortical actin observed in B,D,F,H (triangle) is indicated in A,C,E,F with a white dashed line. Images shown are single CSLM sections taken close to the substrate surface, which is visible in E and G because of the binding of the negatively charged FITC-labelled dextran. Scale bar, 10 µm.





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