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Fig. 3. Pathways of endocytosed fluid-phase marker and resorbed bone matrix converge. Osteoclasts were plated on fluorescently labelled dentine and incubated for 4 hours before the addition of TRITC-labelled dextran 3000 for 30 minutes. After fixation, the distribution of labelled bone matrix (A,C, green) and low-molecular-weight dextran (B,C, red) was analysed with CSLM. Most of the visible vesicular structures contain both labels (C, yellow); the few vesicular structures containing only dextran label are located towards the centre of the cell (C, arrowheads). The outline of the cells as delineated by {alpha}vß3 integrin antibodies is depicted as a dashed white line. Scale bar, 10 µm.





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