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Fig. 5. Lamellipodial and filopodial markers in peripheral processes after SCAR-depletion. Control (A,C) and SCAR-depleted (B,D) S2R+ cells were stained with phalloidin (left panels) and Arp3 (A,B) or Ena (C,D) antibody (middle panels). Merged images are shown in right panels with phalloidin staining in green, and antibody staining in red. Peripheral processes of SCAR-depleted S2R+ cells are weakly stained for Arp3 (B), but no Ena signal is detected (D), in contrast to normal filopodia of BG2 cells which have strong Ena staining at their tips (not shown). Bars, 2 µm.





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