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Fig. 6. MMP-2 is downregulated in AhR–/– MEF. (A) MMP-2 activity was analyzed in conditioned media from AhR+/+ and AhR–/– growing for 72 hours in OPTI-MEM. A gelatinolytic assay was employed as indicated in Materials and Methods. The positions of the pro-(72 kDa), intermediate (64 kDa) and active (62 kDa) MMP-2 are indicated by arrows. Gelatinolytic activity in conditioned media from HT1080 cells was used as control. (B) Mmp-2 mRNA expression was determined by RT-PCR in AhR+/+ and AhR–/– MEF cultures using the primers listed in Table 1. (C) mRNA expression of the MMP inhibitors Timp-1 and Timp-2 was also analyzed by RT-PCR in AhR+/+ and AhR–/– MEF cultures with the primers indicated in Table 1. In panels B and C, ß-actin levels were also analyzed. The experiments were done in duplicate in two different MEF cultures.
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