spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1 Evaluation of the accumulated amount of neurofilament proteins and transcripts in transgenic and control samples. (A) A typical western blot of brain and spinal cord crude extracts from control (–) and transgenic (+) mice was revealed using antibodies against NFH, NFM and NFL. A much lower amount of each subunit was regularly found in transgenic samples. (B-B') The levels of NFHLacZ mRNA (B') were found to be between 12- and 80-fold lower compared with the endogenous NFH mRNA (B). No major difference in the expression of the endogenous NFH gene was observed between control and transgenic mice during ageing. (C) A 10-fold lower level of NFM mRNA was detected in brain compared with spinal cord without significant difference between control and transgenic tissues. (D-D') A similar pattern of expression of NFL was found between control and transgenic samples. In spinal cord, a 4-fold increase of the light transcript (2.5 kb) has been observed between the earlier stage of life and the other stages.





Right arrow Return to article