spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Trypsin proteolysis of neurofilaments isolated from control (WT) and NFHLacZ transgenic (TG) mice. (A) Neurofilaments (25 µg) were incubated during 20 minutes with 20 ng (for NFM) or 4ng (for NFH and NFL) of trypsin at 30°C. The reaction was stopped by the addition of Laemmli buffer, boiled during 5 minutes and analyzed by western blot. (B) 50 µg of neurofilaments were digested for 20 minutes at 37°C with trypsin (2 ng for NFH and NFL, and 4 ng for NFM), then sedimented for 30 minutes at 100,000 g and 4°C. Supernatant (S) and pellet (P) fractions were analysed by western blot. Similar proteolytic patterns and solubility of fragments were observed between control and transgenic mice.





Right arrow Return to article