spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Immunoblot analysis of neurofilaments from control (WT) and NFHLacZ transgenic (TG) mice treated with {alpha}-chymotrypsin. (A) Isolated neurofilaments (10 µg) were treated during 20 minutes with 4 ng of {alpha}-chymotrypsin at 30°C, and then analysed by western blotting. (B) Similarly, neurofilaments (50 µg) were digested for 20 minutes at 37°C with {alpha}-chymotrypsin (2.5 ng for NFH and NFL, or 10 ng for NFM) and centrifuged for 30 minutes at 100,000 g and 4°C. Supernatants (S) and pellets (P) were separated and analysed by western blot.





Right arrow Return to article