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Fig. 3. Expression and colocalization of MIP(AQP0) and Cx45.6 detected by dual-immunostaining of sagittal sections of embryonic-day-10 and -20 lens. (A) Sagittal sections from bow (A-C) and core (D-F) regions of embryonic-day-10 chick lens were prepared and co-immunostained with antibodies specific for MIP(AQP0) and Cx45.6, and subsequently stained with fluorescein-conjugated goat anti-mouse IgG for MIP(AQP0) (A,D) and followed by rhodamine-conjugated goat anti-rabbit IgG for Cx45.6 (B,E). The corresponding images from the same regions were merged together to demonstrate the overlapping patterns between these two proteins (C,F). Scale bar, 20 µm. (B) Sagittal sections from embryonic-day-20 chick lens were similarly prepared and co-immunostained with anti-MIP(AQP0) monoclonal antibody (A,D) and affinity-purified anti-Cx45.6 (B,E) antibodies. The merged images were shown in (C,F). Scale bar, 20 µm.
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