spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. C-terminus of MIP(AQP0) pulled down lens-fiber connexins from chick embryonic-day-10 lens lysates. Ni-NTA beads conjugated with six-histidine-tagged C-terminus of MIP(AQP0) were used to pull down proteins from lens lysates. Total lens lysates (lane 1) and retained fractions of lysate by Ni-NTA beads in the absence (lane 2) or presence (lane 3) of fusion protein were visualized by Coomassie Blue staining. The retained fraction of lysate by fusion-protein-conjugated-beads (lanes 4 and 7), the flow-through factions after extraction by fusion-protein/Ni-NTA beads (lanes 5 and 8) and total lens lysate samples (lanes 6 and 9) were immunoblotted with affinity-purified anti-Cx45.6 antibody (lanes 4-6) and anti-Cx56 antibody (lanes 7-9).





Right arrow Return to article