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Fig. 7. Transient interactions of MIP(AQP0) with lens-fiber connexins at bow regions of adult lenses. Lens sections prepared from 1-month-old chicken were double labeled with monoclonal anti-MIP(AQP0) antibody and affinity-purified anti-Cx45.6 antibody, and subsequently labeled with fluorescein-conjugated goat anti-mouse IgG (A,D,G,J) for MIP(AQP0) and followed by rhodamine-conjugated goat anti-rabbit IgG for Cx45.6 (B,E,H,K). The corresponding images from the same regions were merged together to demonstrate the overlapping patterns between these two proteins (C,F,I,L). (A-C) A region at the lens bow regions; (D-F) a region at the anterior part of the lens fibers; (G-I) a region at the posterior part of the lens fibers; (J-L) a region at the center core of the lens fibers. Scale bar, 20 µm.





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