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Fig. 3. Seven-day bovine articular cartilage labelled with antibody to Notch 1 (A) and counterstained with propidium iodide. Chondrocytes within the uppermost 2-3 cell layers of the surface zone (arrows) label strongly for Notch 1. Chondrocytes were labelled with anti-notch 1 antibody and subjected to single-channel FACS analysis immediately after isolation (B). 86% of surface zone cells label positively for N1 compared with 10% and 34% from middle and deep zone, respectively. (*P<0.001 compared with middle and deep.) Chondrocytes were selected immunomagnetically and subjected to differential adhesion and initial adhesion and CFE assessed. Notch 1-selected surface zone cells (SFN N1) were more adherent than N1-selected middle (MFN N1) and deep zone (DFN N1) cells and unselected cells (SFN 20, MFN 20, DFN 20) (C). In addition, the CFE of surface zone cells selected for N1 was greater than notch-positive middle and deep zone cells and unselected cells (D). *P<0.001 compared with middle and deep, **P<0.001 compared with N1 selected and unselected middle and deep cells, ***P<0.01 compared with unselected surface zone cells, ****P<0.01 compared with selected and unselected middle and deep zone cells. Abbreviations as in Table 1.





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