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Fig. 1. Mutants tested in plasmid segregation assays. (A) Schematic representation of cnx1 constructs tested. The hatched box represents the highly conserved central domain; dotted line represents deleted sequences; dark grey box next to SP represents N-terminal domain; light grey box following hatched box (or dotted line) represents C-terminal domain within the ER lumen; ADEL represents the ADEL ER-retention signal of S. pombe; black boxes, potential sites of phosphorylation by protein kinase C (PKC); SP, signal peptide of Cnx1p; TM, transmembrane domain. (B) Plasmid segregation assays were carried out by culturing the cnx1 + pcnx1 + pcnx1 cells in liquid non-selective medium and scoring auxothrophies/protothropies on solid media as described in Materials and Methods. For each line, the first construct mentioned is on the pREP41 (LEU2 marker) vector and the second construct is on pREP42 vector (ura4⁺ marker). When only one plasmid is denoted, the cnx1 construct is based on the pREP41 vector. ^a, Average of at least two independent experiments; ^b, average of at least three experiments.

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