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Fig. 3. Cin and Cin + pcnx1+ cells display temperature-sensitive growth. Cells were cultured to saturation and then diluted into 10 ml of fresh MM+Ade+Ura medium to OD595 of 0.02. (A) Growth curves of SP556 (genomic cnx1+), SP3220: cnx1{Delta} + pcnx1+, SP7188: Cin and SP7202: Cin + pcnx1+ cells at 30°C for 45 hours. (B) Growth curves of the same cells grown at 37°C for 72 hours. (C) Histograms of calculated growth rates for each strain, at 30°C and 37°C.





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