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Fig. 4. Cin cells display thermosensitive altered morphology. (A-C) S. pombe cells exponentially grown at 30°C were incubated for 20 hours in MM+Ade+Ura (2% glucose) at 30°C (A), or at 37°C (B). Cells were then stained with the fluorescent dye Calcofluor White (upper part of A and B) and viewed with Nomarski interference (lower part of panels A and B). Arrows indicate intracellular accumulation of the fluorescent dye. (C) Confocal indirect immunofluorescence analysis on cnx1+ (SP3220: cnx1{Delta} + pcnx1+), Cin (SP7188) and Cin + pcnx1+ (SP7202) cells was carried out with anti-Cnx1p rabbit antibodies (as described in Materials and Methods). For the Cin strain, anti-BiP antibodies were used for the endoplasmic reticulum immunostaining.





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