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Fig. 5. The Cin state can be transmitted by transformation and requires a proteinaceous factor. Extracts from Cin (SP7188) and cnx1{Delta} + pcnx1+ (SP3220) cells were prepared and treated with RNaseA, DNaseI and UV, as described in Materials and Methods. Cell extracts were treated with proteinase K, or untreated, as indicated. Five and 15 µg of these extracts were transformed into strain SP3220 (cnx1{Delta} + pcnx1+) using the PEG/LiAc method. After plasmid segregation assay the appearance of Cin cells was scored. The table on the right gives details about the numbers of colonies tested. The frequencies of Cin cells are the mean of two to three independent experiments. In initial experiments, the cell extracts were fractionated by centrifugation into pellet and soluble fractions. Since both the soluble and pellet fractions of Cin (SP7188) cell extracts transmitted the Cin state, transformations were carried out with unfractionated lysates.





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