spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Co-localisation of the Ca2+-ATPase ACA4 and m-Rabmc with the PVC and the GA. Secondary antibodies used were as described in Fig. 3. Images were colour-coded with Adobe Photoshop. Single confocal images of isolated BY-2 cells. Scale bars: 5 µm. (A-C) Single image of a cell co-labelled with ACA4-Cy3/BP80-FITC. (A) ACA4 staining; (B) BP80 staining; (C) merged image reveals an almost complete co-localisation of ACA4 and BP80 (yellow). (D-F) Single image of a cell co-labelled with ACA4-FITC/JIM84-Cy3. (D) ACA4 labelling; (E) labelling of the GA recognised by JIM84; (F) merged image of the dual labelling. Note some co-localisation (yellow arrowheads, inset). (G-I) Image stack of a single cell labelled with ACA4-FITC/m-Rabmc-Cy3. The secondary Cy3-coupled antibody was a F(ab')2 fragment. (G) ACA4-labelling; (H) m-Rabmc labelling; (I) merged images. Note the almost complete co-localisation of ACA4 and m-Rabmc (yellow).





Right arrow Return to article