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Fig. 6. Effect of submicromolar free Ca²⁺ on SNARE complexes in permeabilized PC12 cells. (A) SNAP-25-immunoreactive bands from samples extracted from PC12 cells that had been exposed to intracellular buffer containing 100, 250, 500 or 1000 nM free Ca²⁺ in the presence of 10 µM digitonin for 20 minutes before membrane preparation. (B) Comparison of the levels of SNAP-25-immunoreactive proteins in membrane extracts of cells permeabilized in the presence of 100 nM and 1000 nM free Ca²⁺. Data are from three independent experiments. Levels of the 230 kDa band (light grey bar) and the 100 kDa band (dark grey bar) observed in the presence of 1000 nM free Ca²⁺ are shown as percentage (± s.d.) of the signals obtained with 100 nM free Ca²⁺.

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