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Fig. 3. Cells depleted of ini1+ arrest in the G2/M phase of the cell cycle. (A) DAPI-stained {Delta}ini1 cells containing the wild-type ini1+ gene under the control of the thiamine repressible promoter Rep81 ({Delta}ini1), grown in the absence (left panel) or presence (right panel) of thiamine. (B) Cell number increase for {Delta}ini1 in the presence or absence of thiamine. (C) DNA content of the cells described in A, 12 hours after addition of thiamine.





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