QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. LAT is required for its own TCR-induced recruitment to the immune synapse. (A) Transiently transfected LATWT-, LAT ${Delta}$ Ct- and LATY/F-GFP Jurkat cells were added to CellTracker^TM Orange-labelled APCs for 3 minutes. Cells were then fixed and analysed by confocal microscopy. Histograms depict the percentage of T cells, after 3 minutes or 7 minutes of contact with SEE-pulsed APCs, showing either a clear clustering of LATWT-GFP (left panel) or a polarization of the intracellular LAT-containing compartment towards the APC (right panel). Quantification was performed on more than 35 conjugates for each LAT construct. Results of one representative experiment out of two are shown. Bar, 5 µm. (B) GFP fluorescence of LATWT-, LAT ${Delta}$ Ct-, LATY/F- and LAT ${Delta}$ 32-104-GFP in JCAM2.5 cells interacting with SEE-pulsed Raji B cells. JCAM2.5 transiently reconstituted with the four LAT constructs were added to CMTMR-labelled (red) Raji B cells and shortly centrifuged to synchronize the contacts. After 8 minutes, cells were fixed and analysed by confocal microscopy. White arrowheads point to the site of initial contact and white stars show the LAT-containing intracellular compartment when visible. (C) Histograms depict the percentage of T cells showing either a clear clustering of LATWT-GFP (grey) or a polarization of the intracellular LAT-containing compartment towards the APC (black). Quantification was performed blindly on 15 conjugates for each LAT construct. Black star indicates the weak intracellular expression of LAT ${Delta}$ 32-104-GFP, such that polarization could not be properly assessed. Bar, 5 µm.

Return to article