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Fig. 5. Inhibition of Rab7 affects the SCV membrane. HeLa cells were transfected with vectors encoding GFP-tagged wild-type Rab7, Rab7-T22N (dominant negative variant), or RILP-C33 (truncated Rab7-binding variant of RILP) prior to infection with S. typhimurium. (A) Representative confocal micrographs showing LAMP-1 distribution (red in merged images) in GFP-Rab7-, GFP-Rab7-T22N-, and GFP-RILP-C33-expressing cells infected for 8 hours by GFP-S. typhimurium (WT) or GFP-sifA mutant strains (bacteria are green in merged images, GFP-tagged Rab7, Rab7-T22N and RILP are not visible because of significantly lower overall fluorescence intensity). Scale bar: 5 µm. (B) LAMP-1 association with bacteria in HeLa cells infected for 8 hours with GFP-S. typhimurium (white bars) or the GFP-sifA mutant (grey bars). LAMP-1 association was assessed by confocal microscopy. Results shown are the mean ± s.d. of at least three independent experiments in which more than 100 bacteria were analysed for each experiment.





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