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Fig. 1. Knockdown of each topo II isoform by siRNA. (A,B) HeLa cells were transfected with {alpha} siRNA-1 (A1), {alpha} siRNA-2 (A2), ß siRNA-1 (B1) and ß siRNA-2 (B2) or buffer as a control (A and B; C). Cells were assayed on the second, third and fourth days after transfection by immunoblotting using the anti-topo II{alpha} antibody 8D2 (left panels) and the anti-topo IIß antibody 3B6 (right panels). (C) Gene silencing was also confirmed by immunofluorescence using the anti-topo II{alpha} antibody 8D2 (for {alpha} siRNA-1-knockdown cells, upper panels) or the anti-topo IIß antibody 1A5 (for ß siRNA-2-knockdown cells, lower panels); cells were also stained with DAPI. Ab: antibody. Bar, 20 µm.





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