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Fig. 6. Chromosome condensation and chromosome axis shortening. (A) Metaphase chromosomes of control (a,b), topo II{alpha}-knockdown (c,d) and topo II{alpha}ß-knockdown (e,f) cells were spread and stained with DAPI (a,c,e) and the anti-topo II{alpha}ß antibody 7B9 (b,f) or the anti-topo II{alpha} antibody 8D2 (d). In control cells, chromosome axes and centromeres were stained with anti-topo II{alpha}ß antibody. Bar, 10 µm. (B) Frequency distribution of the length of metaphase chromosomes in control cells and topo II{alpha}ß-knockdown cells.





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