Movie 1 Horizontal
-
Movie 1. Epidermal
cells were isolated, labeled with the red fluorescent dye CM-DiI and injected
into diabetic mice with an ischemic leg. One month after the injection, mice
were perfused with a FITC-BSBL4 lectin (green, specific for mouse endothelial
cells) before tissue was harvested. Because the lectin labels only the luminal
side of the endothelial cells, the cells would appear to wrap around the
labeled lumen. Pictures were taken with a confocal microscope and
three-dimensional reconstruction performed digitally using Metamorph® software
to ensure that cells truly wrap around the lumen, and do not simply appear to
do so because of the angle of section. Note that after rotation (vertical or
horizontal), some red fluorescent cells (epidermal cells CM-DiI cells)
incorporated into the vasculature (delineated by the green fluorescence), i.e.
they ‘rotated with the vessel’, whereas lots of other red cells did not.
