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Fig. 2. Incorporation of freshly isolated epidermal cells into the vasculature. Freshly isolated epidermal progenitor cells (A,B) and transient amplifying cells (C) were labeled with CM-DiI (red fluorescent dye) and injected into a mouse ischemic hindlimb. Two weeks later, biopsies of skin (A,D) and ischemic muscles (B,C,E) were immunostained for CD31 (to identify endothelial cells; green) and keratin 14 (blue, a marker for epidermal basal cells; A, arrowheads). CM-DiI-labeled (red) cells, co-labeled CM-DiI and anti-CD31 (yellow) cells, but not keratin-14-positive (blue) cells can be seen in the vessels. Arrows indicate two capillaries enlarged in (D). No injected cells (red) could be seen in the vasculature (green) in the contralateral control muscle (E). Notice that the two red dots in (A) correspond to autofluorescent hair shaft cut in oblique sections and are not CM-DiI-injected cells. Scale bars, 25 µm.

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