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Fig. 1. Nucleo-somatodendritic translocation of Sam68 in depolarized rat hippocampal neurons. (A) Immunostaining of Sam68 using the AD1 rabbit anti-serum in neurons at 9 DIV cultured at 5 mM KCl showing a nuclear labeling (left) or at 25 mM KCl showing a perinuclear staining or a somatodendritic staining (right, see arrows). (B) Confocal image of a same neuron cultured at 25 mM KCl labeled with the anti-Sam68 AD1. Sam68 was concentrated in clusters (arrowheads, red fluorescence, upper panel) and MAP2 antibodies (green fluorescence, lower panel).





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