spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Association of GFP-Sam68 granules with RNA. (A) Confocal images of dendritic localization of GFP-Sam68 in hippocampal neurons showing fluorescent granules (green fluorescence, GFP-Sam68) that colocalize in three regions (yellow fluorescence, merged image) with RNA labeling by ethidium bromide (red fluorescence). Percentage of colocalization of GFP-Sam68 and ethidium bromide. Error bar represents s.d. (n=100). (B) RNAse I treatment of the EtBr labeling of neurons. (C) DNAse I treatment of the EtBr labeling of neurons. (D) Binding of immunoprecipitated Sam68 from adult rat cortex with ß-actin mRNA. RT-PCR with specific ß-actin primers was carried out with AD1 anti-Sam68 antibody immunoprecipitation (lane 1), with preimmune IgG immunoprecipitation (lane 2), with total extract (lane 3) or with no input (lane 4).





Right arrow Return to article