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Fig. 5. Dendritic microtubule-dependent transport of Sam68 during depolarization. (A) Effect in percent of a microtubule-disrupting drug vincristine (5 µM) in depolarized neurons. GFP-Sam68{Delta}CREs-transfected neurons were incubated with 25 mM KCl for 5 hours in the absence (hatched) or presence (stippled) of vincristine. Error bars represent s.d. (n=3, where 200-300 transfected cells were counted). (B) Images of the {alpha}-tubulin immunostaining in depolarized neurons in the absence or presence of vincristine. (C) Images of the GFP-Sam68 expression pattern in depolarized neurons in the absence or presence of vincristine.





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