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Fig. 2. Cell surface localization of CEACAM1-L mutants. MDCK cells stably transfected with mouse wild-type CEACAM1-L and CEACAM1-L mutants were grown to confluent, polarized cell layers on permeable filters. Cell layers were fixed, permeabilized, and analyzed by confocal microscopy with antibodies against mouse CEACAM1. A z-reconstruction (top) and an x,y-field between the apical and the basal cell surfaces (bottom) are shown for each CEACAM1-L variant. Lateral cell surface staining is seen in wild-type CEACAM1-L and in the mutants {Delta}518, Y488F and V518A, whereas mutants {Delta}510, {Delta}495, {Delta}472, {Delta}461, Y515F, Y488,515F, 3K-3A and S503A lack lateral staining for CEACAM1-L. CEACAM1-positive intracellular vesicles are seen in several of the cells.





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