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Fig. 4. Effects of pervanadate on CEACAM1-L Tyr phosphorylation. Untransfected MDCK cells and MDCK cells stably transfected with rat CEACAM1-L or CEACAM1-S were grown to confluence in Petri dishes and treated with pervanadate (100 µM) for the indicated times. The cells were then analyzed by immunoblot for CEACAM1 expression and Tyr-phosphorylation of CEACAM1 using antibody against CEACAM1 ({alpha}-CEACAM1) and antibody against phosphorylated-Tyr ({alpha}-PY), respectively. Maximal Tyr-phosphorylation of CEACAM1-L occurred after 4 min. CEACAM1-S was not Tyr-phosphorylated.





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