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Fig. 7. Colocalization of CEACAM1-L with adaptor proteins AP-1 and AP-2. Confluent CEACAM1-L-expressing cells were double-stained for CEACAM1-L (red) and AP-1 (green) or AP-2 (green). Single focal x,y-planes from within CEACAM1-L-expressing cells, recorded above the level of the nucleus are shown. Bars, 10 µm. (A) Cells cultured on coverslips. (Left) AP-1 was closely associated with CEACAM1-L in structures that were observed predominantly within the cells (encircled area). (Right) AP-2 colocalized with CEACAM1-L in the plasma membrane (encircled area) and in vesicular structures close to the plasma membrane (encircled area). (B) Cells cultured on filters. In cells treated with pervanadate for 2 minutes, prominent colocalization of CEACAM1-L and AP-1 was seen in large vesicular structures (left). In cells treated with staurosporine for 60 minutes, clusters of small vesicular structures occurred that showed close association of CEACAM1-L and AP-2 (right).
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