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Fig. 8. Localization of CEACAM1-L in relation to occludin, ZO-1, E-cadherin and ß-catenin. Confluent, filter-grown polarized cells expressing rat CEACAM1-L were fixed, permeabilized and analyzed using confocal microscopy. Staining is shown in individual and merged vertical z-reconstructions and in merged horizontal x,y-planes. Arrowheads indicate the position of the z-reconstruction in the corresponding x,y-plane, and the position of the x,y-plane in the corresponding z-reconstruction. (Top group of panels) Staining for occludin (green), ZO-1 (green) and CEACAM1-L (red). No colocalization of CEACAM1-L with either occludin or ZO-1 was observed. (Bottom group of panels) Staining for E-cadherin (green), ß-catenin (green) and CEACAM1-L (red). Notice the prominent colocalization (yellow) of CEACAM1-L with E-cadherin and ß-catenin in the apical parts of the lateral cell surfaces at sites corresponding to adherens junctions. Bars, 10 µm.





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