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Fig. 1. Kinetics of internalisation and recycling of VSG and endocytosis of fluid-phase marker. (A) Rate of internalisation. VSGbiotin uptake is expressed as the fraction of surface-associated VSGbiotin prior to endocytosis (t=0). The data represent the average (±s.e.) of five independent experiments. (B) Rate of recycling. Exocytosis of VSG is expressed as the non-acetylated VSG transported from the cell interior to the acetylated surface normalised to the total surface VSG without treatment. The data represent the average (±s.e.) of five independent measurements. (C) Rate of fluid-phase uptake of Alexa Fluor 488 dextran. Internalisation is expressed as the change in relative fluorescence intensity/cell. Data from three different experiments performed at a range of concentrations (1.25 to 5 mg/ml) were normalised to 1 mg/ml and averaged.





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