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Fig. 3. Time-resolved, quantitative co-localisation analysis of VSG during endocytosis. Increase of co-localisation or of segregation in intracellular space was described empirically by first-order processes of the form Ax(1-e^-kxt), and where this was followed by a second slower phase that derived from the first (D,E), by Ax(l-e^-kxt)x{1+Bx[1-e^-hxt]}. A represents the amplitude of the first phase, B is a measure for the efficiency at which A contributes to the extent of the second phase, and k and h are the rate constants. Values in brackets in E were not obtained independently from the data, but were fixed as taken from the corresponding values in D. VSG_biotin gains access to intracellular compartments as defined by clathrin (A), RAB5 (B), RAB7 (C) or RAB11 (D). The kinetics of the percentage of co-localisation between endocytosed VSG_biotin and the four markers are shown for cells taken from the same experiment. (E) Segregation of endocytosed VSG_biotin from Alexa Fluor 488-conjugated dextran.

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