JCS00939 Movies

Files in this Data Supplement:

• Movie 1 -

  Movie 1. (see Fig. 1Cd-f) Preferential localization of APC to a subset of centrioles. A 360° counterclockwise rotation around the x-axis of the centrosome region of a U-2 OS cell co-immunostained for centrin as a marker for centrioles (green) and APC (red). Sections of this region were deconvolved and recombined in a 3D rendering. The rotation shows localization of APC to one of four centrioles as a U-shaped tube that is closed at one end and more open at the other end (see also Movie 4). APC also extends to a second centriole, closely aligning the centrin core along its length.

• Movie 2 -

  Movie 2. (see Fig. 2Ca-c) Preferential localization of GFP-APC to one of two centrioles. A 360° counterclockwise rotation around the x-axis of the centrosome region of a U-2 OS cell expressing GFP-APC (green) and immunostained for centrin as a marker for centrioles (red). Sections of this region were deconvolved and recombined in a 3D rendering. The rotation shows preferential localization of GFP-APC (green) to one of two centrioles (red).

• Movie 3 -

  Movie 3. (see Fig. 5A and A˘) EB1 localizes to the mother centriole. 360° counterclockwise rotations around the x-axis of a centrosome region of a nocodazole-treated U-2 OS cell expressing GFP-centrin as a marker for centrioles (blue) and coimmunostained for EB1 (red) and for the mother centriole with e-tubulin (green). Sections of this region were deconvolved and recombined in a 3D rendering. The first 360° rotation shows EB1 (red) localization to one of two centrioles. The second 360° rotation shows e-tubulin (green) localization to the mother centriole. The third 360° rotation shows EB1 (red) and e-tubulin (green) co-localization to the same end of the mother centriole. EB1 (red) caps this end of the mother centriole like a wizard’s hat, whereas e-tubulin forms a more ring-like structure around the same end.

• Movie 4 -

  Movie 4. (see Fig. 6B and B˘) APC localizes to the mother centriole. 360° counterclockwise rotations around the x-axis of a centrosome region of a U-2 OS cell expressing GFP-centrin as a marker for centrioles (blue) and coimmunostained for APC (green) and for the mother centriole with p150^Glued/dynactin (red). Sections of this region were deconvolved and recombined in a 3D rendering. The first 360° rotation shows APC (green) tightly surrounds one of four centrioles and extends onto a second centriole. The two central centrioles are too close to each other to be resolved as separate spots. The second 360° rotation shows p150^Glued/dynactin (red) localization to the mother centriole. The third 360° rotation shows APC (green) and p150^Glued/dynactin (red) co-localization to the mother centriole. APC extends to a second centriole without p150^Glued/dynactin indicating that APC localization to the new centrosome precedes that of p150^Glued/dynactin.

• Movie 5 -

  Movie 5. (see Fig. 6C and C˘) EB1 and p150^Glued/dynactin cap the same end of the mother centriole and extend out onto microtubules from this end. 360° counterclockwise rotations around the x-axis of the centrosome region of a U-2 OS cell expressing GFP-centrin as a marker for centrioles (blue) and coimmunostained for EB1 (green) and the mother centriole with p150^Glued/dynactin (red). Sections of this region were deconvolved and recombined in a 3D rendering. The first 360° rotation shows EB1 (green) localization to one of two centrioles. The second 360° rotation shows p150^Glued/dynactin (red) localization to the mother centriole. The third 360° rotation shows EB1 (green) and p150^Glued/dynactin (red) co-localization to the mother centriole. EB1 (green) and p150^Glued/dynactin (red) cap one end of the mother centriole and continue in a MT-like filamentous extension from this end of the centriole.