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Fig. 5. Spontaneous Ca²⁺ sparks in ^wtRyR3- and ^V2322DRyR3-expressing HEK293 cells. (A,B) Individual traces show time courses of spontaneous Ca²⁺ sparks in ^wtRyR3- and ^V2322DRyR3-expressing cells, respectively. The contour images show the corresponding regions over which the relative changes in fluorescence were integrated. (C) The bar chart shows that the percentage sparking was significantly larger in the ^V2322DRyR3- than in the ^wtRyR3-expressing cells (68.3±3.8 versus 44.4±2.7, n=18, P<0.001). (D-F) The Ca²⁺-spark properties, mean amplitude and duration were significantly smaller in the ^V2322DRyR3-expressing than in the ^wtRyR3-expressing cells whereas the spark frequency was not affected. Amplitude (A) expressed as % ${Delta}$ F/F: 40.2±1.3, n=109 versus 48.6±2.0, n=82 in the ^V2322DRyR3- and ^wtRyR3-expressing cells (P<0.001), respectively. Duration (seconds): 0.37±0.02, n=109 versus 0.56±0.06, n=82 in the ^V2322DRyR3- and ^wtRyR3-expressing cells (P=0.001), respectively. Frequency (per second): 1.62±0.23, n=12 versus 1.29±0.16, n=12 in the ^V2322DRyR3- and ^wtRyR3-expressing cells, respectively (P=0.24).

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