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Fig. 6. FKBP12 overexpression in wtRyR3- and V2322DRyR3-expressing HEK293 cells. (A) FKBP12 expression levels were determined in the homogenate of wtRyR3- and V2322DRyR3-expressing HEK293 cells after exogenous overexpression of FKBP12 by western blot analysis, using the polyclonal FKBP12 antibody (1/1000). FKBP12 expression was increased by 2.07±0.27 (n=4) in wtRyR3-expressing cells and 1.63±0.22 (n=4) in V2322DRyR3-expressing cells. All lanes contained 30 µg protein. (B) The bar chart shows that the percentage sparking was significantly reduced in the wtRyR3 cells overexpressing FKBP12 (44.4±2.7, n=18 versus 5.3±2.7, n=7, P<0.001) while FKBP12 overexpression did not significantly affect the percentage sparking in V2322DRyR3-expressing cells (68.3±3.8, n=18 versus 57.7±3.2, n=10, P<0.068).





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