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Fig. 4. Identification of the syntaxin 5 cleavage sites. (A) Schematic representation of the structure of syntaxin 5 isoforms showing the putative caspase-3 cleavage sites. Transmembrane domains are indicated in black and coiled coil-forming regions are shaded grey. The actual cleavage site is underlined. (B) In vitro translated 35S-labelled wild-type syntaxin 5 or syntaxin 5 with the indicated aspartic acid to alanine substitutions were incubated with control or apoptotic HeLa cytosol in the absence or presence of 2 µM Ac-DEVD-CHO for 4 hours at 37°C and analysed by SDS-PAGE and autoradiography. Full-length (FL) and caspase cleavage products (*) are marked. Note that the single point mutations give rise to a slight shift in mobility of the full-length protein.





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