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Fig. 7. VSV-G-GFP accumulates in the endoplasmic reticulum of apoptotic cells. (A) HeLa cells were transfected with tsO45 VSV-G-GFP, incubated overnight at 39.5°C and shifted to 31°C for 25 minutes. After fixation and permeabilisation, cells were stained with antibodies to the Golgi apparatus (GM130). Scale bar, 10 µm. (B) Transfected HeLa cells were incubated in the presence of 2 µM staurosporine for an additional 4 hours at 39.5°C to induce apoptosis prior to shifting to 31°C for 25 minutes. Cells were fixed, permeabilised and stained with the ER marker calnexin (red) and DAPI (blue). VSV-G-GFP is green and regions of overlap with calnexin are yellow. The images are three separate confocal slices taken through the middle region of the cell. Scale bar, 10 µm.
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