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Fig. 3. PDGF-BB expression in the fetal gonad. (A) Northern-blot analysis, performed on 20 µg of total RNA extracted from 12.5 and 13.5 dpc male and female gonads. An erythroleukaemic cell line (K562) was used as a positive control, which, after treatment with TPA expresses high levels of PDGF-BB mRNA; a human lymphoma cell line (MOLT-4) was used as a negative control. Lower panel: filter reprobed with ß-actin cDNA to check for the presence of equal amounts of mRNA in each lane. (B) Immunoblot analysis showing a PDGF-BB immunoreactive band of 30 kDa, detected in a nonreducing gel, which represents the PDGF-BB dimeric molecule present in fetal testis.





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