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Fig. 9. Effect of U0126 and Ly294002 on proliferation and migration of mesonephric mesenchymal cells. (A) Quiescent cells were pretreated with U0126 (U0) or Ly294002 (Ly) and then stimulated with PDGF-BB. [³H]TdR incorporation was measured as an index of cell proliferation. Results are means±s.d of four independent experiments, each done in quadruplicate. ^*P<0.01 vs its respective control (no inhibitor). (B) Quiescent cells were pretreated with U0126 or Ly294002 and then tested for directed migration towards 10 ng/ml PDGF-BB. Results are means±s.d. of four independent experiments, each done in duplicate. ^*P<0.01 vs PDGF-BB 10 ng/ml.

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