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Fig. 4. Effects of wild-type and bas TAL-1 overexpression on cell proliferation and migration. (A) 7x10⁴ HUVECs were seeded onto collagen-coated 12-well plates and infected with Ad-Tet-Off and with either Ad-LacZ or Ad-wtTAL-1 or Ad-bas and cultured in complete medium. Cell numbers were monitored at 72 hours post-infection by direct counting. The number of LacZ-infected ECs was arbitrarily set at 100. The values are the mean±s.d. of independent infection experiments (n=10); statistical significance relative to Ad-LacZ infected cells is indicated: ^*P<0.05 (Student's t-test). (B) HUVECs or UCB-ECs were co-infected with Ad-Tet-Off and with Ad-TRE-wtTAL-1, Ad-TRE-bas or Ad-TRE-LacZ. Twenty hours post-infection, 4x10⁴ cells were placed in the upper wells of 8 µm cell culture inserts in serum-free medium. The lower wells contained either control medium or chemoattractant medium. After 90 minutes, cells were fixed and labeled with propidium iodide. Migration was quantified by counting the cells that had migrated through the inserts in 6 randomly selected fields (original magnification, x10). The number of cells that had migrated through the inserts towards a serum-free medium was considered as background and was arbitrarily set at 1. The values are the mean±s.d. of three independent infection experiments; statistical significance relative to Ad-LacZ infected cells is indicated: ^*P<0.05; ^**P<0.01; ^***P<0.001 (Student's t-test).

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