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Fig. 5. Effects of wild-type and 
bas TAL-1 ectopic expression on in vivo angiogenesis. Male SCID mice were injected subcutaneously at three sites with 0.5 ml Matrigel mixed with bFGF, heparin and Ad-Tet-Off together with each Ad-TRE. Injection sites were chosen such that each animal received the three Ad-TRE-LacZ, wtTAL-1 and bas. Three experiments were carried out with five or six animals. Matrigel plugs were removed after 6 days, photographed and processed for histology. Macroscopic observations. Representative photographs of three Matrigel implants formulated with the indicated adenovirus harvested from two individual mice belonging to two different experimental groups. Note the increase in angiogenesis (as demonstrated by brown/red color) in wt-TAL-1-transduced implants and its inhibition in -bas-transduced implants (almost white) as compared to control LacZ implants. Histological analyses. (Top) Representative images of Matrigel plug sections after Hematoxylin and Eosin staining; capillary structures with lumen (indicated by arrows) were more numerous in wtTAL-1 and very rare in bas as compared to control implants (LacZ). Higher magnification (x20) shows the presence of red blood cells within these structures only in wtTAL-1- and LacZ- but not in bas-formulated implants. (Bottom) Low magnification image (x5) illustrating the very large vascular structures with large vascular cavities engorged with red blood cells observed in wt-TAL-transduced implants. (C) Immunohistochemical staining with anti-CD31 antibody. Representative photographs illustrating the infiltration of CD31-positive ECs (appearing in brown) and their different organization within the three types of Matrigel implants.
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