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Fig. 4. Cross sections of testes from adult wild-type (A,D) and Lmna–/– (B,C,E,F) mice. Sections are stained with Hematoxylin (A,B,D,E) or in situ labeled using the TUNEL assay and counterstained with Methyl Green (C,F). (A-C) Stage V, (D-F) stage VII in the epithelium cycle. (G) Transmission electron microscopy of a seminiferous tubule from a 22-day-old Lmna–/– mouse. S, Sertoli cell, P, pachytene spermatocyte. Arrows in E and arrowheads in G denote some of the dead spermatocytes. (F) Higher magnification of a dead spermatocyte. N, nucleus, C, cytoplasm. Arrows denote SC structures. Scale bars: 20 µm (A-G), 0.5 µm (H).





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