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Fig. 6. Spread spermatocytes of 28-day-old Lmna–/– (A-C) and heterozygous (D) littermates stained for the axial element protein SCP3 (green, FITC) and the telomere protein Trf1 (red, rhodamine). Complete synaptonemal complexes (SCs; thick green rods) display Trf1 signals (red dots) at their termini as do the numerous long unpaired axial elements. (A) Zygotene-like Lmna–/– spermatocyte with numerous unpaired axes. (B) Zygotene-like Lmna–/– spermatocyte with a short univalent and numerous unpaired axes. (C) Lmna–/– spermatocyte at pachytene exhibits univalent X (arrow) and Y chromosomes (long arrow). (D) Pachytene spermatocyte of a heterozygous control littermate with normal SCs and a sex chromosome bivalent (arrowhead) with Trf1 signals at all telomeres. Scale bars: 10 µm.





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