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Fig. 2. Schematic representation of the 3D arrangement of GRPCWM in the protoxylem of soybean hypocotyls. (A) Differentiating PX at the top of the hypocotyl. (B) Mature protoxylem in the middle of the hypocotyl. Green shows GRP-containing structures. Red shows lignified secondary wall thickenings. Differentiation of the cells occurred from left to right. Therefore, the oldest protoxylem element is located at the left and the metaxylem element (m) at the right. A comparison between the two oldest PX in (A,B) shows their passive elongation and the continued deposition of GRPCWM in the dead cells. The nonextensible metaxylem elements do not contain GRP, except in the cell corners shared with protoxylem elements.





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