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Fig. 4. Enzymatic digestion of isolated soybean PX. Isolated PX with widely spaced ring-shaped SW are compared after different treatments. (A,D) Buffer control. (B,E) Cellulase. (C,F) Proteinase K. (A-C) Phase contrast microscopy; arrows indicate GRPCWM. (D-F) Epifluorescence microscopy of the same PX as shown in (A-C). The arrows indicating GRPCWM are located at the same positions as in (A-C). The ring-shaped SW remain connected after all three treatments. However, the intensity of the fluorescence is reduced after the treatment with proteinase K. Bars, 20 µm.





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